ELISA lecture2004.pptVIP

ELISA AND RELATED TECHNOLOGIES ENZYME-LINKED IMMUNOSORBENT ASSAY COATING THE PLATE WASHING THE PLATE BLOCKING THE PLATE SAMPLE CONJUGATE AMPLIFICATION amplify with or SUBSTRATES MICROPARTICLE ENZYME IMMUNOASSAY (ABBOTT AxSYM) ALLERGEN-SPECIFIC IgE: Pharmacia UNICAP system Detection of cytokines by ELISA DETECTION OF CYTOKINE-SECRETING CELLS BY ELISPOT ASSAY PBM + stimulus, 22-24 hr WASH Add biotin-labelled detection anti-CK. 90 min. Wash Add streptavidin-alkaline phosphatase. 60 min. Wash Colourless, soluble BCIP-NBTinsoluble blue product CK ELISPOTS - NORMAL RANGES (n = 60)(CK-secret