11基因芯片2007.ppt

* 1)用基因表达芯片探索药物作用机理 * 探索药物作用机理 treatment with oestrogen and tamoxifen它莫西芬 (a selective oestrogen receptor modulator). Pilot experiments were done first to determine the optimal duration of treatment, and we found that a 3-hr treatment of cEECs内皮细胞瘤 with 17b-oestradiol (E2) yielded the greatest coverage (about 75%) of currently known ER target genes. Thus, we chose a 3-hr treatment for subsequent experiments. * Endometrioid carcinoma samples (cEECs) and from samples of normal endometrial epithelium (nEECs) * 作用机理 Nature Review,2006,6 * 它莫西芬的代谢通路 * 基因调控的复杂性(多层次) Information Functional