牛乳腺炎金黄色葡萄球弹性纤维结合蛋白N端蛋白nEBPS基因的.docVIP

稿号：2012-225，收稿日期：2012-5-3 牛乳腺炎金黄色葡萄球菌弹性纤维结合蛋白N端蛋白nEBPS基因的克隆及序列分析 薛晓阳1、2，吴金花1，布日额1**，王学理2，刘洋1、2，刘燕1，西林高娃1，孙立杰1，郭闯1，薛江东2 （1.内蒙古民族大学生命科学学院，内蒙古通辽 028043；2. 内蒙古民族大学动物科技学院，内蒙古通辽 028043） 【摘要】根据Genbank上公布的金黄色葡萄球菌弹性纤维结合蛋白N端蛋白nEBPS基因的全序列，利用生物学软件Primer5.0和Oligo 6.0设计了一对特异性引物，采用PCR方法扩增了内蒙古分离株的nEBPS基因序列。结果表明，扩增的基因片段长度为720bp， 与标准菌株（CMCC26074）的nEBPS基因片段序列相似性为100%， 与GenBank上公布的金黄色葡萄球菌菌株（U48826.2）nEBPS基因相似性达到97.36%，为进一步研究该致病菌的发病机制和建立分子检测技术奠定了一定的实验基础。 【关键词】金黄色葡萄球菌；nEBPS基因；克隆；序列分析 Cloning and sequencing of fibronectin flexibble N-terminal protein nEBPS gene of Streptococcus aureus from mastitis cows XUE Xiao-yang1、2, WU Jin-hua1, BU Ri-e1,WANG Xue li2, LIU Yang1、2 , XI Lin gao wa1, SUN Li jie1, GUO Chuang1, XUE Jiang dong2 （1. College of Life Science, Inner Mongolia University for Nationalities ，Tongliao 028043, Inner Mongolia, China; 2.College of Animal Science and Technology, Inner Mongolia University for Nationalities） 【Abstract】A pair of primers were designed by biological software of primer 5.0 and Oligo 6.0 according to fibronectin?flexible?N-terminal?protein?nEBPS gene sequences of bovine mastitis Staphylococcus aureus published on GenBank. nEBPS gene fragment of clinical isolated strain was amplified by PCR. Sequencing result showed that nEBPS gene sequences had 720bp in length. nEBPS gene sequences of clinical isolated strain had 100% of homology with the standard strain（CMCC26074） in gene sequences, and had 97.36% of homology with the EBPS gene sequences of standard S. aureus strain (U48826.2) published on GenBank. The bovine mastitis Staphylococcus aureus nEBPS gene had been cloned and sequenced. This result had laied a foundation for further study on the pathogenic mechanism and establishment of a molecular detecting method for Staphylococcus aureus in dairy milk. 【 Key words】 Staphylococcus aureus；nEBPS gene；cloning; sequencing *【基金项目】国家自然科学基金项目（No；内蒙古自治区科技厅科技创新引导计划项目（2011年、2012年）。 **【通讯作者】布日额，Tel：0475-8314626，E-mai