Recombinant DNA technology 2016.pptVIP

Recombinant DNA technology : There are the methods used to accomplish DNA cloning. We also call it genetic engineering . purpose : ① isolate an interested gene or DNA sequence----cloning ② get product (protein) of interested gene----expression has a suitable origin of replication ； contains one or more selectable markers that allow the easy identification of the host cells harboring them ； has single restriction sites at which foreign DNA can be inserted; it is easy for them to entry into host cells because of their small size