Bacillus.pptVIP

Bacillus sp. GP rod Spore former Examples B. anthracis B. cereus B. subtilis B. megatarium Exoenzymes Lipase Amylase Casease Gelatinase Spores Collagen Hydrolysis Enzyme Gelatinase Use loop (vs. needle) Inoculate several times Stab deep Substrate Gelatin Reaction Clear Zone on Agar Liquefy Gelatin in tube Nitrate Reduction Inoculate with loop Incubate Additional solutions Sulfanilic acid (A) Naphthylamine (B) (+) = Red Nitrate to Nitrite No color, add Zinc (+) = not red (-) = red Results Exoenzymes Starch (amylase, maltase) Lipid (lipase) Proteins (Casease, protease) Collagen (Gelatinase) Rea