2016消化科开题报告.pptVIP

Tim3，即T细胞免疫球蛋白及粘附素结构域蛋白3 表达在Th1细胞表面，是其标志分子 与其配体Galectin9结合致Th1细胞减少，调节免疫反应 材料及方法 试验动物: Tim-3 transgenic mice Galectin-9 transgenic mice Tim-3?/? mice Rag1?/? C57BL/6 mice:无T和B细胞 Galectin-9 transgenic and Tim-3?/? mice EAE模型：实验性自身免疫性脑脊髓炎injected subcutaneously with 100 μg of MOG 35–55 peptide (MEVGWYRSPFSRVVHLYRNGK) emulsified in CFA (Difco) supplemented with 4 μg ml?1 Mycobacterium tuberculosis and injected twice intravenously with 100 ng of pertussis toxin (List Biological Laboratories). Notes CD44highCD62Llow：effector/memory T cell CD44lowCD62Lhigh ：naive cell CD11b+ ：m