DNAreplication改.pptVIP

3. Removal of RNA primer by DNA pol I with 5’-3’ exonuclease activity on lagging strand 4. Filling the gap by DNA pol I with 5’-3’ extension activity 5. Ligation of Okazaki fragments are linked by DNA ligase. Termination and Segregation 1.The two replication forks meet approximately 180o opposite oriC. Around this region are several terminator sites which arrest the movement of the forks by binding the tus gene product,an inhibitor of the DnaB helicase. Termination and Segregation 2.When replication is completed, topoisomerase IV is to unlink the interlinked daughter genomes. Replicatio