Basics f Cell Culture细胞培养.pptVIP

Safety aspect in cell culture Possibly keep cultures free of antibiotics in order to be able to recognize the contamination Never use the same media bottle for different cell lines. If caps are dropped or bottles touched unconditionally touched, replace them with new ones Necks of glass bottles prefer heat at least for 60 secs at a temperature of 200 C Switch on the laminar flow cabinet 20 mts prior to start working Cell cultures which are frequently used should be subcultered stored as duplicate strains 藤攫掺能晕锁苗怜碱擂阮改司木炔禽窑修腮敦碰亩丫酣墨凉汕腆奎舵砍版Basics of Cell Culture细胞培养Basics of Cell Culture细胞培养 Other key facts…….? Use actively growing cells that are in their log phase of growth, which are 80-90% viable Keep exposure to trypsin at a minimum Handle the cells gently. Do not centrifuge cells at high speed or roughly re-suspend the cells Feeding sub culturing the cells at more frequent intervals then used with serum containing conditions may be necessary A lower concentration of 104cells/ml to initiate subculture of rapidly growing cells a higher concentration of 105cells/mlfor slowing growing cells 豫菜侦婆孙淬键泼鄙醒慰疤靳很吻咐雨介侯垛匹庆箕侨漓塌肯自辟逮砒裙Basics of Cell Culture细胞培养Basics of Cell Culture细胞培养 Thanks 入醒申吹韩肘痛防绢耕坦掌乡洼税钝量废乏陷送撕呕扬岭射剔怕亚挑蓖墨Basics of Cell Culture细胞培养Basics of Cell Culture细胞培养 * * * * * * * * * * * * * * * * * * * * * * * * Paras Yadav1, Annu Yadav1, P. Kumar1, J.S. Arora1, T.K.Datta1, S. De1, S.L. Goswami1, Mukesh Yadav2, Shalini Jain3, Ravinder Nagpal4 and Hariom Yadav3 1Department of Animal Biotechnology, 3Animal Biochemistry Division and 4Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001 (Haryana), India; 2SOS in Chemistry, Jiwaji University, Gwalior-474011, M.P., India Basics of Cell Culture 舱暑高冰舅作坊揽铭碾而初冲靠馁糯陋雁腺催庆牢愁骸构橇怂原妈屯讳篱Basics of Cell Culture细胞培养Basics of Cell Culture细胞培养 Introduction Cell culture is the process by which prokaryotic, eukaryotic or plant cells are grown under controlled conditions. But in practice it refers to the