基因工程6-1表达载体2cmx解剖.ppt

10 10 EcoR I Pst I ATG TAG Sal I Sma I 题2 克隆表达 37℃,1h 37℃,1h Vector Gene 14 ℃, 6h or overnight, then store at -20 ℃ Protocol Cell 37℃, 45min Transform Protocol 37℃, 16-20h PCR, Sequence, Express 30-32℃,200rpm,OD600=0.4-0.8 42℃，200r/min,3-5h 37℃,200rpm, OD600=0.4-0.8 42℃,200rpm, 3-5h purification Protocol pGEX-6P-3 肠激酶 37℃,1h 37℃,1h Vector Gene 14 ℃, 6h or overnight, then store at -20 ℃ Protocol Cell 37℃, 45min Transform Protocol 37℃, 16-20h PCR, Sequence, Express 37℃,200rpm,OD600=0.4-0.8 IPTG(1 mmol / L)，200r/min,3-5h 37℃,200rpm, OD600=0.4-0.8 IPTG(1 mmol / L)，200r/min,3-