BioSci145ALecture112122002Transgenictechnology课程.pptVIP

BioSci 145A Lecture 11 - 2/12/2002 Transgenic technology and its implications Topics we will cover today how to get the DNA into cultured cells. chemical transfection of cultured cells cell fusion (won’t discuss) liposome-mediated transfer cationic dendrimers virus mediated electroporation how to get DNA into embryos biolistic gene transfer microinjection electrotransformation viral infection transgenic technology standard transgenesis gene targeting Dr. La Morte will discuss single cell microinjection techniques How to get DNA into cells - introduction General terminology transformation r