ExpressionSystems课程.pptVIP

Expression Systems 1 Bacterial Expression Systems 2 Baculoviral Expression Systems 3 Additional Expression Systems 4 Heterologous(异种的) Expression in Mammalian Cells 5 Transfection Methods 6 Cotransfection of Several Genes 7 Transient and Stable Transfections 8 Reporter Genes Lecture 7 Prokaryotic expression systems 7.1. pQE expression system in E.coli 7.2. pMAL Expression system in E.coli  T7 expression vector: ---ori---ampR---T7promoter---RBS-ATG-MCS-Transcription-terminator--- RBS stands for ribosome binding sequence. pQE expression vector*: ---ori---ampR---T5promoter---RBS-ATG-hexaH