海水培养光细菌存在的问题及其优化研究.docVIP

海水培养光合细菌存在的问题及其优化研究 摘要 [目的]为光合细菌海水培养技术的大规模应用提供理论依据。[方法]采用对比试验研究光合细菌在不同培养基中的生长状况,探讨光合细菌在海水培养过程中出现沉降现象的原因,在此基础上对培养基组分进行优化并进行优化培养试验。[结果]在海水培养光合细菌的过程中,酵母膏、蛋白胨培养基中沉降现象较轻,其余培养基中培养2 d后均出现较严重的沉淀现象,主要是因为培养过程中培养基的pH值逐渐升高,其中的磷酸盐与海水中的Ca2+和Mg2+结合成沉淀,从而使细菌附着在沉淀表面生长所致。优化后的培养基组分为:乙酸钠3.0 g/L,氯化铵1.0 g/L,磷酸二氢钾0.1 g/L,酵母浸膏0.5 g/L。[结论]用优化后的培养基培养光合细菌效果理想,培养7d后光合细菌的个数可达3.16@109个/ml。 关键词 光合细菌;海水;培养 Problem of Photosynthetic Bacteria Culture in Seawater and Optimization of Culture Conditions Abstract [Objective] The aimwas to provide theoretical basis for the application of culture technology for photosynthetic bacteria in seawater in large scale. [Method] The contrast testwas taken to study the growth status of photosynthetic bacteria in differentmediums and to explore the reason of photo- synthetic bacteria appearing subsidence phenomenon inseawater in cultureprocess. Onthebasisof it, themediumcomponentswereoptimizedandtheop- timized culturewas carried out. [Result] In the process of photosynthetic bacteria culture in seawater, the subsidence phenomenon in yeast extract and peptone mediumwas slight and that in othermediumswas serious after cultured for2 d.Themainreasonwas that, withthe graduallyrise of pHvalue of medium in culture process, the phosphate combined into precipitation with Ca2+andMg2+in seawater, so the bacteria attached to the precipitation to grown.. The optimized mediumwas that sodiumacetate of 3.0 g/L, chloride of 1.0 g/L, potassium dihydrogen phosphate 0.1 g/L, yeast extract 0.5 g/L. [Conclusion] The culture effect of photosynthetic bacteriawith optimized mediumwas ideal, and the number of photosynthetic bacteria could reach 3.16@109ind/ml after cultured for 7 d. Key words Photosynthetic bacteria; Seawater; Culture 光合细菌(Photosynthetic Bacteria,PSB)是一类能在厌氧光 照或好氧黑暗条件下利用有机物作供氧体兼碳源,进行不放氧 光合作用的有益细菌,不仅可作为光合作用机理和产氢固氮的 研究材料[1-2],且在养殖业、种植业、环境治理和新能源开发利 用等领域具有广阔的应用前景[3-8]。在水产养殖中,光合细菌 可降解水体中的有机质和硫化物等有毒物质,实现其充当饵 料、净化水质、预防疾病和作为饲料添加剂等的功能,因此光合 细菌在无公害水产养殖中具有巨大的应用价值[10]。目前生产