细胞工厂研究进展学习课件.pptVIP

An introduction to cell factory Background The increasing shortage of fossil resource and global climate change today demand fundamental innovation and new concepts in bioprocess development for the production of commodities (chemicals) and fuels from renewable resources. Currently, bioproduction of commodities and fuels in large scale is almost exclusively based on microbial systems, primarily in form of pure culture. Background In 1982.the concept of biorefinery was first proposed in Science .aims at step-wise fractionation/conversion to achieve efficient separation of major components. Since then, the biorefinery becoming the strategic direction of the world. Significant progress 2009, Ishchuk et. al raised the fermentation temperature of an ATHI mutant strain of Hansenula polymorpha yeast to 50 ℃, by excessive co-expression the heat shock proteins, 16p and 104p . 2010, SuwrannaraIlgsee et. al increased ethanol production of H. polymorpha by over express alcoholdehydrogellase (ADH) derived from itself , (up to 36.7g/L). Significant progress In Netherlands, Delft University of Technology selected a engineering strain limited by xylose, and resulted in a strain which can efficiently digest xylose mixed by glucose. From this mixed culture, a high ethanol productivity of 0. 49g/g was achieved. Significant progress Wisselink et. al screened out the strains that can fully consume the solution, 30 glucose, 15 g xylose, 15g Yasar Arabia of sugar (per litre), and shorten the fermentation time 40%, by evolutionary engineering. Significant progress By the manipulating of metabolic engineering, Succinic acid productivity of the engineering strain was reach at 1. 29mol/mol glucose. Xylose isomerase (木糖异构酶), xylulokinase (木酮糖激酶), transketolase (转酮酶) was expressed in Zymomonas. mobilis. The production of ethanol was enhanced about 44.7% from this recombined strain. Significant progress The yields of ethanol was increased by 19% result from truncated SacB(果聚糖蔗糖酶) gene of Z