AllinceUPLC系统使用与维护导论.ppt

I have this as backup in case it comes up! This slide demonstrates that with new users we are continuing to find bacteria in column frits, usually from bad water or other source of contamination. Poor solvent grades and not filtering buffers (although we have used successfully 200 mM Sulphate buffers routinely, still have to remind users not to then shutoff pump). The learning curve or the re-adoption of Good Lab Practices is short, but can be difficult. GLP is less critical for columns that have 2.0 um frits, but is essential for columns with 0.2 um frits and short, small ID tubing. Class　A：33号 Class　B：51号 I have this as backup in case it comes up! This slide demonstrates that with new users we are continuing to find bacteria in column frits, usually from bad water or other source of contamination. Poor solvent grades and not filtering buffers (although we have used successfully 200 mM Sulphate buffers routinely, still have to remind users not to then shutoff pump). The learning curve or the re-adoption of Good Lab Practices is short, but can be difficult. GLP is less critical for columns that have 2.0 um frits, but is essential for columns with 0.2 um frits and short, small ID tubing. I have this as backup in case it comes up! This slide demonstrates that with new users we are continuing to find bacteria in column frits, usually from bad water or other source of contamination. Poor solvent grades and not filtering buffers (although we have used successfully 200 mM Sulphate buffers routinely, still have to remind users not to then shutoff pump). The learning curve or the re-adoption of Good Lab Practices is short, but can be difficult. GLP is less critical for columns that have 2.0 um frits, but is essential for columns with 0.2 um frits and short, small ID tubing. Column efficiency or plate count and HETP are common terms used with column and system performance. The flow rate of mobile phase also affects the plate count value. Flow rate and test marker also have