原发性支气管肺癌中PTEN基因的蛋白表达及意义.docVIP

原发性支气管肺癌中PTEN基因的蛋白表达及意义 　　作者：李雅莉，辛丽红，杨侠，张洁，李家芝，刘云 作者单位：西安交通大学医学院第二附属医院呼吸内科，陕西西安 710004 　　【摘要】 目的 研究抑癌基因PTEN在原发性肺癌中的蛋白表达及其临床意义。方法 应用免疫组织化学SP法检测56例手术切除的肺癌石蜡标本及3例正常肺组织中的PTEN基因的蛋白表达情况。结果 ①正常肺组织中PTEN蛋白表达的阳性率明显高于肺癌患者，而在小细胞肺癌中的阳性表达低于非小细胞肺癌;②在56例肺癌患者中，随着分化程度的降低，PTEN的蛋白阳性表达率也随之下降;③Ⅰ、Ⅱ期肺癌患者的PTEN蛋白阳性表达率高于Ⅲ、Ⅳ期患者;④在19例有转移的肺癌患者中，PTEN表达阳性率明显低于无转移的患者。结论 PTEN基因的表达与肺癌的病理类型、组织的分化程度、临床分期以及有无淋巴结转移密切相关。 　　【关键词】 肺癌;PTEN基因;免疫组织化学 　　Expression of PTEN protein in primary lung cancer 　　Li Yali, Xin Lihong, Yang Xia, Zhang Jie, Li Jiazhi, Liu Yun 　　(1. Department of Respiration, the Second Affiliated Hospital,Medical School of Xian Jiaotong University, Xian 710004;2. Department of Respiration, Xian Childrens Hospital, Xian 710002, China) 　　ABSTRACT: Objective To study the protein expression level and clinicalsignificance of the tumor suppressor gene PTEN in primary lung cancer. Methods We detected the protein expression of PTEN gene in paraffin specimens of 56 cases of lung cancer and 3 specimens of normal lung tissues by SPimmunohistochemistry. Results ① The positive rate of PTEN protein expression was higher in normal lung tissues than in lung cancer specimens, while it was lower in SCLC than in NSCLC. ② The positive rate of expression of PTEN protein became lower as the differentiation level decreased in the 56 cases of lung cancer specimens. ③ The positive rate of expression of PTEN protein was higher in patients with lung cancer of stage Ⅰ and Ⅱ than that in stage Ⅲ and Ⅳ. ④ The positive rate of expression of PTEN protein was 26.32% in 19 cases of transfused specimens, which was obviously lower than that in non-transfused specimens. Conclusion The expression of PTEN gene is related to classification criteria of lung cancer, malignancy degree, clinical stage and lymph node transfusion. 　　KEY WORDS: lung cancer; phosphatase and tensin homologue deleted from chromosome 10 gene(PTEN); immunohistochemistry 　　肿瘤的发生是多阶段、多步骤和多基因改变的过程，肿瘤的恶性表型是多种因素相互作用导致正常细胞恶变的结果，