实验九质粒DNA的制备和酶切.pptVIP

Restriction Endonucleases:? An Overview Restriction enzymes were discovered about 30 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Bacteria initially resist infections by new viruses, and this restriction of viral growth stemmed from endonucleases within the cells that destroy foreign DNA molecules. Among the first of these restriction enzymes to be purified were EcoR?I and EcoR II from Escherichia coli, and Hind II and Hind III from Haemophilus influenzae. These enzymes were found to cleave DNA at specific sites, gener