- 1、本文档共37页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Restriction Endonucleases:? An Overview Restriction enzymes were discovered about 30 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Bacteria initially resist infections by new viruses, and this restriction of viral growth stemmed from endonucleases within the cells that destroy foreign DNA molecules. Among the first of these restriction enzymes to be purified were EcoR?I and EcoR II from Escherichia coli, and Hind II and Hind III from Haemophilus influenzae. These enzymes were found to cleave DNA at specific sites, generating discrete, gene-size fragments that could be re-joined in the laboratory. Researchers were quick to recognize that restriction enzymes provided them with a remarkable new tool for investigating gene organization, function and expression. As the use of restriction enzymes spread among molecular biologists in the late 1970’s, companies such as New England Biolabs began to search for more. Except for certain viruses, restriction enzymes were found only within prokaryotes. Many thousands of bacteria and archae have now been screened for their presence. Analysis of sequenced prokaryotic genomes indicates that they are common--all free-living bacteria and archaea appear to code for them. Restriction enzymes are exceedingly varied; they range in size from the diminutive Pvu II (157 amino acids) to the giant Cje I (1250 amino acids) and beyond. Among over 3,000 activities that have been purified and characterized, more than 250 different sequence-specificities have been discovered. Of these, over 30% were discovered and characterized at New England Biolabs. The search for new specificities continues, both biochemically, by the analysis of cell-extracts, and computationally, by the analysis of sequenced genomes. Although most activities encountered today turn out to be duplicates--isoschizomers--of existing specificities, restriction enzymes with new specificities are found with
您可能关注的文档
- 实训五、WEB服务器的配置与管理.ppt
- 实用 电容的识别与检测.ppt
- 实训四 国电器的供应链管理改善方案终稿.docx
- 实训八、DHCP服务器的安装与配置.ppt
- 实训任务431RLC串联电路特性的测试和分析.ppt
- 实训报告册2.ppt
- 实训报告任书.doc
- 实践决定认识.ppt
- 实践报告第页格式、第二页目录.docx
- 实训任务41 正弦交流信号的测试.ppt
- 剧本杀行业报告:内容创作规范与剧本市场拓展策略.docx
- 剧本杀行业区域市场区域文化特色与市场潜力分析报告.docx
- 剧本杀行业区域市场拓展实战案例研究.docx
- 剧本杀行业区域市场拓展路径与模式探索报告.docx
- 剧本杀行业区域市场竞争态势与品牌差异化策略研究报告.docx
- 剧本杀行业2025年西北区域市场市场细分领域竞争态势与品牌竞争策略分析研究报告.docx
- 剧本杀行业2025年西北市场拓展前景预测报告.docx
- 剧本杀行业2025年长沙市场发展潜力分析报告.docx
- 剧本杀行业2025年长三角市场竞争策略与布局分析.docx
- 医疗行业数据合规:2025年数据安全法实施后的合规监管挑战与应对.docx
最近下载
- 《第一单元 生活中的音乐学习项目二 音乐开启心灵之窗》教案(表格式)七年级音乐人教版(2024新版).pdf VIP
- 成功标书大餐——15份浙江省自然科学基金申请书.pdf VIP
- (辽宁富士电梯)系统故障代码及说明.doc VIP
- 2025版高考政治全程一轮复习必修2第一单元生产资料所有制与经济体制第一课我国的生产资料所有制课件.pptx VIP
- 陕西省专业技术人员继续教育2025公需课《党的二十届三中全会精神解读与高质量发展》20学时题库及答案.docx VIP
- 伤口换药护理操作.pptx VIP
- 贵州01-贵州省居住建筑节能设计标准DBJ52-49-20082.pptx VIP
- 《2023年度中国展览数据统计报告》.docx
- 城市名居商住楼房地产估价报告.doc VIP
- [说明]富士变频器维修与故障处理.doc VIP
文档评论(0)