黄芪总黄酮对病毒性心肌炎小鼠心脏血流动力学及心肌细胞钙电流的作用.docVIP

【摘要】 目的 研究黄芪总黄酮(TFA)对BALB/c小鼠病毒性心肌炎急性期心脏血流动力学及心肌细胞钙电流的作用。方法 应用柯萨奇B3病毒制作BALB/c小鼠病毒性心肌炎模型7只，腹腔注射TFA溶液（20 mg/kg)1次/d，并设正常对照组及病毒感染对照组各7只，7 d后行血流动力学检查；应用胶原酶酶解法急性分离病毒性心肌炎BALB/c小鼠心室肌细胞，利用全细胞膜片钳的方法记录TFA对BALB/c小鼠病毒性心肌炎模型心室肌细胞Ca2+电流的作用。结果 （1）TFA组与正常对照组和病毒感染组相比血流动力学指标明显改善。（2）TFA组与对照组比较，L型钙电流(LICa)由给药前的(0.28±1.07) nA增加到给药后的(0.39±0.84) nA(P0.05)。结论 TFA可以明显改善BALB/c小鼠病毒性心肌炎急性期心脏血流动力学，TFA可增加病毒性心肌炎BALB/c小鼠心室肌细胞LICa的幅值。 【关键词】 黄芪； 心肌炎； 肌细胞，心脏 Effects of total flavonoids of astragalus mongholicus on cardiac hemodynamics and calcium current of ventricular myocytes in BALB/c mouse with viral myocarditis LIU Guannan*, CHEN Guoquan, ZHAO Ming. *Department of Pediatrics, Tongliao City Kerqin District Hospital, Tongliao 028000, China Corresponding author: ZHAO Ming, Email: langzhe73@ 【Abstract】 Objective To study the effects of total flavonoids of astragalus mongholicus (TFA) on cardiac hemodynamics and calcium current of ventricular myocytes in BALB/c mouse with viral myocarditis. Methods Twentyone BALB/c mice were randomly divided into 3 groups, control group (n=7), virus infection control group (n=7), and TFA group (TFA intraperitoneal injection, 20 mg/kg, n=7). All the mice were sacrificed on day 7 and the changes of left ventricular pressure (LVP) and rate of change of left ventricular pressure (LV dp/dt) were measured, meanwhile the Langendorff perfusion method was used to isolate the ventricular myocytes and calcium current was recorded using patch clamp technic. Results Compared with that of virus infection control group, the parameters of cardiac hemodynamics in TFA group was significantly improved. The calcium current was elevated from (0.28±1.07) nA to (0.39±0.84) nA in TFA group (P=0.0089). Conclusions TFA can significantly improve cardiac hemodynamics and enhance Lcalcium current of ventricular myocytes in BALB/c mouse with viral myocarditis. 【Key words】 Astragalus membranaceus; Myocarditis; Myocytes, cardiac 中药黄芪具有显著的益气强心作用。在临床上除