蕨麻对急性低压缺氧大鼠脑组织保护作用及capase 3表达的影响(学报).docVIP

蕨麻对急性低压缺氧大鼠脑组织保护作用及caspase 3表达的影响 杨 虎1，张永亮2,3*，龚海英2，李灵芝2,3*，高宏生2,3 (1武警河北总队医院病理科，河北 石家庄 050081；2武警后勤学院，天津 300309；3天津市职业与环境危害防制重点实验室，天津 300309) 摘要：【目的】研究蕨麻对模拟高原低压缺氧环境下大鼠脑组织capase 3表达的影响。【方法】78只健康成年雄性SD大鼠随机分为常氧对照组、低压缺氧组、蕨麻乙醇提取物高[3.6 g.(kg.d)-1]、中[1.8 g.(kg.d)-1]、低[0.9g.(kg.d)-1]剂量组和尼莫地平[0.02g.(kg.d)-1]组，每组13只。除常氧对照组外，其余各组大鼠均在减压舱内模拟海拔7000m缺氧12h。测定脑含水量变化；H.E染色观察脑皮质病理改变；透射电镜观察脑皮质神经元超微结构变化； RT-PCR和免疫组织化学法检测大鼠脑组织Caspase-3 mRNA及其蛋白的表达。【结果】与低压缺氧组相比，蕨麻乙醇提取物[3.6g.(kg.d)-1] 组大鼠脑皮质形态结构接近常氧对照组,超微结构损伤显著减轻；大鼠脑组织的含水量显著降低；大鼠脑皮质Caspase-3 mRNA及其蛋白的表达明显下调(P0.01)。【结论】蕨麻乙醇提取物能够减轻低压缺氧导致的脑水肿，保护脑组织，此作用可能与其下调脑组织Caspase-3表达，抑制神经细胞凋亡有关。 关键词:低压缺氧；蕨麻；脑水肿；半胱氨酸天冬氨酸蛋白酶he brain protective effect of Potentilla anserina L and its regulating on expression of caspase-3 in rats exposed to acute hypobaric hypoxia YANG Hu, ZHANG Yong-liang, Gong Hai-ying, LI Ling-zhi, Gao Hong-sheng(Logistics University of Chinses People’s Armed Police Force; Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental hazard, Tianjin 300162, China) Abstract:【Objective】To study the brain protective effect of Potentilla anserina L and its regulating on expression of caspase-3 in rats exposed to acute hypobaric hypoxia.【Methods】78 Male Sprague-Dawley Rats were randomly divided into control group, acute hypobaric hypoxia group, intervention groups of Potentilla anserina L. alcohol extract [3.6g.(kg.d)-1, 1.8g.(kg.d)-1, 0.9g.(kg.d)-1] and Nimodipine group[0.02g.(kg.d)-1], and each group with 13 rats. Pathological and ultrastructural changes in the cerebral cortex were observed respectively by HE staining and transmission electron microscope，the brain water content was determined RT-PCR and immunohistochemistry assays were used for detecting the caspase-3 mRNA and protein.【Result】Compared with control group，the morphological strcture were improved and the ultrostructure injury were reduced in cortex of rats treated with 3.6g.(kg.d)-1 Potentilla anserina L. alcohol extract. The brain water content red