SMART 技术构建肾乳头状腺癌及癌旁组织的cDNA文库.docVIP

SMART 技术构建肾乳头状腺癌及癌旁组织的cDNA文库 作者：成瑜 李旭 陈葳 杨玉琮 【关键词】 肾肿瘤 Construction of cDNA libraries of renal papillary adenocarcinoma tissue and its side tissue with SMART technology 【Abstract】 AIM: To construct the cDNA libraries of renal papillary adenocarcinoma tissue and its side tissue by SMART technology. METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as