三七总皂甙对梗阻性黄疸肝组织中bax，bcl.docVIP

三七总皂甙对梗阻性黄疸肝组织中bax，bcl 作者：周斌，于博，姚南，宋少莉 【关键词】 阻塞性黄疸；,bax；,bcl 【Abstract】 AIM：To explore the protective mechanism of Panax notoginoside against hepatic injury after obstructive jaundice in rabbits. METHODS：Fortyeight healthy Japanese white rabbits were randomized into 2 groups, 24 in each group: group A treated with common bile duct ligation , group B of BDL + PNS. All animals were kept under the same condition. Immunohistochemistry was used to determine the expressions of Bax and Bcl2 in hepatic tissues. RESULTS： In group A, expressions of bcl2 and bax in the hepatic tissue were observed after OJ. Expression of bax reached the peak at the 9th day after OJ, then tended to decline. Similarly, the highest expression of bcl2 was observed at the 21th day after OJ. The apoptosis index increased with the increase of bax expression but decreased with bcl2 strong expression. After treatment with PNS in group B, a lower expression of bax and a higher expression of bcl2 were found, and the AI value was also lower, compared with group A. CONCLUSION： The pathology of OJ may be related to the initiation of apoptosis. PNS may play a protective role against hepatic injury after OJ, which may be realized through upregulating the expression of bcl2 and downregulating the expression of bax in [三七总皂甙对梗阻性黄疸肝组织中bax，bclhttp:// 欢迎您访问范.文,之.家]the hepatic tissues. 【Keywords】 obstructive jaundice; bax; bcl2; Panax notoginoside;liver; rabbits 【摘要】 目的：探讨梗阻性黄疸肝损伤中的病理调控机制及三七总皂甙的保护. 方法：健康日本大耳白兔48只，雌雄各半，随机分为2组，每组24只. A组：胆总管接扎组；B组：BDL+PNS组. 建立实验动物模型. 所有实验动物均在相同条件下饲养. 采用免疫组化法检测肝组织bax，bcl2的表达情况. 结果： A组动物在胆道梗阻后，肝组织可见bcl2和bax蛋白表达， bax蛋白在梗阻9 d达高峰，此后开始下降. 而bcl2蛋白在梗阻21 d达高峰. 凋亡指数随bax升高而升高，随bcl2的升高而降低；B组动物bax表达较同时相A组弱，而bcl2蛋白强于A组，B组AI值较A组降低. 结论：梗阻性黄疸所致的肝组织病理变化可能与凋亡程序的启动有关. PNS对梗阻性黄疸肝组织损伤有明显的保护作用,其机制可能是与上调肝组织bcl2蛋白及下调bax蛋白有关. 【关键词】 阻塞性黄疸； bax； bcl2； 三七总皂甙；肝；兔 0引言 梗阻性黄疸是外科的常见病症，肝脏是最先受损并损伤最重的器官. 本实验我们通过检测梗阻性黄疸大耳白兔肝组织bax，bcl2蛋