人膀胱逼尿肌中肾上腺素能β3受体基因的克隆及其载体的构建.docVIP

人膀胱逼尿肌中肾上腺素能β3受体基因的克隆及其载体的构建 作者：李昕,王平,李刚,刘屹立 【关键词】 受体;,肾上腺素能β3;,载体 β3AR gene cloning from human bladder detrusor smooth muscle and construction of its antisense eukaryotic expression vector 【Abstract】 AIM: To clone human beta3 adrenoceptor gene from human bladder detrusor smooth muscle and construct its antisense eukaryotic expression vector. METHODS: The β3AR fulllength cDNA was cloned and amplified from human detrusor muscle cells through RTPCR and subcloned into clone vector . The target gene was then cut from ClaI/HindIII s