精子上游器的研制及其实验效果.docVIP

精子上游器的研制及其实验效果 　　【摘要】目的：研制一种新型精子优选器皿―精子上游器（发明专利号：ZL 20081），并检测其实验效果。方法：采用精液样本30份，每份精液样本平分为两份，分别用精子上游器（实验组）和传统的四试管上游法（对照组）上游处理，然后分析处理后精子的浓度和活力及前向运动精子数。结果：实验组精液a级、前向运动精子率和前向运动精子数均高于对照组，差异有统计学意义（P值均0.001）。实验组精子浓度低于对照组，差异有统计学意义（P0.05）。结论：本项目研制的精子上游器使用方便、易于操作，满足辅助生殖技术的临床要求，值得推广应用。 　　【关键词】精子上游器； 精子浓度；精子活力 　　Development of sperm swim-up device and its laboratory efficacyZHANG Peng1， LI Juntao2， LU Xilan2， XU Xiangling2， ZHANG Haiyan2， ZHANG Jianmin3， WANG Hengcai2△. 1. Graduate School of Weifang Medical University， Weifang 261053， China； 2. Department of Reproductive Medicine， Jinan Central Hospital Affiliated to Shandong University， Jinan 250013， China； 3. Department of Reproductive Medicine， Maternity Hospital of Shandong， Jinan 250001， China 　　【Abstract】Objectives： To develop a new type of spermatozoa sorting instrument -sperm swim-up device- （whose invention and patent No. is ZL 20081） and test its experimental effect. Method： 30 semen samples were selected， each being divided into two equal parts and processed by sperm swim-up device （case group） or traditional four-tube-swim-up method （control group） respectively. After that we contrasted and analyzed the sperm concentration， motility and forward movement sperm count of the 2 groups. Results： The percentages of grade a， forward movement spermatozoon and forward movement sperm count in experimental group were all higher than those in the control group； the differences were all statistically significant （P0.001）； The sperm concentration of experimental group was lower than that in the control group， with a statistically significant difference （P0.05）. Conclusion： The sperm swim-up device developed in the project is easy to use and operate， which meets the clinical requirement of assisted reproductive technology and is worthy of popularization and application. 　　【Key words】Sperm swim-up device； Sperm concentration； Sperm motility 　　【中图分类号】R321.1【文献标志码】A近年来，不孕不育的患病率越来越高，在中国约累及10%-15%的育龄夫妇，其中