aml中的分子学监测.pptVIP

All mutations occurred as an insertion of four nucleotides at position 960, except for type VII, which had an insertion at position 964. Method: The PCR products were purified and directly cloned into pGEM-T or pGEM-T-EASYvector. The plasmids were sequenced and the wild-type (WT) and mutant clones were used for standard curves in subsequent real-time PCR. The seven mutants were serially diluted from 106 to 10 copies per reaction and found as low as 10 copies of all mutants could be reliably detected. We used BM genomic DNA without NPM1 mutations to serially dilute the genomic DNA obtained from