Corn genetics Lab Gulf Coast State College玉米遗传学实验室墨西哥湾沿岸州立大学.pptVIP

Corn Genetics Chi- Square Goodness of Fit Test Pedigrees and probability analysis to follow. Outline Highlight some key points in syllabus Corn color and collection of data (phenotypes) Chi-Square Analysis Corn genetics Lab Application of Chi-Square Analysis The color pattern in corn is due to multiple genes Are variations in number of observed phenotypes due to chance alone? Objective Complete corn worksheet Set up Punnett Squares using the genotypes given Compare expected data to observed data Perform Chi Square Analysis Variation of colors—Why? Corn Kernel Color Color of aleurone masks endosperm color Only with colorless aleurone can endosperm color be determined Determined by several genes Refer to genotypes in lab manual! Gene interaction can occur:Epistasis is a form of gene interaction in which one gene masks the phenotypic expression of another. Today there are: 3 crosses of corn  Multiple alleles are tested for expression in F2 generation F1 X F1 Note color differences Cross C Cross D (darker color is purple) Cross F (call purple, may look red!) Cross C F1 phenotype F1 X F1 genotypes Yellow YYC’Rr X YYC’Rr Cross D F1 phenotype F1 X F1 genotypes Purple yyPpRr X yyPpRr Cross F F1 phenotype F1 X F1 genotypes Purple yyCcRr X yyCcRr Chi Square Statistical “Goodness of Fit Test” Why do we use it? . To find out determine if probability are due to chance alone. An example of how to calculate a Chi Square Application of Chi Square Genetic information regarding the corn color patterns are provided in your lab manual.  CORN WORKSHEET IS DUE 3/5