COPD炎症和抗炎治疗例析.ppt

* * Am. J. Respir. Crit. Care Med., Vol 150, No. 5, Nov 1994, 1453-1455. * * * Ottonello L, Morone P, Dapino P, Dallegri F. Inhibitory effect of salmeterol on the respiratory burst of adherent human neutrophils. Clin Exp Immunol 1996: 106: 97-102. ? Blackwell Science * * Wilcoxon signed-rank test. * * * Salmeterol and FP suppress IL-8 release induced by CSM neutrophils were isolated from buffy coats and pretreated for 90 min with FP 10–11–10–7 M (a) or salmeterol 10–9–10–5 M (b) and then stimulated for 9 h with CSM (0.06 OD) The supernatants were harvested and subjected to ELISA methods as described on “Materials and methods”. Data shown are mean±SEM of three independent experiments. Asterisk indicates significant differences between medium-treated cells and cells treated with CSM (***p0.0001) and number sign represents the significance between cells treated with CSM alone and CSM with S or FP (#p0.05 and ##p0.01). S and FP indicate salmeterol and FP, respectively Fig. 4. Effect of fluticasone on cigarette smoke medium-induced IL-8 release. Macrophages were pretreated with fluticasone for 40 min (A) and 2.5 h (B) prior to incubation with cigarette smoke medium (0.06 OD) for 16 h. IL-8 accumulation in the free cells medium was measured by ELISA as described in material and method. (?Pb0.05) compared to cigarette smoke medium stimulated cells. * Meta-analysis of the effect of inhaled corticosteroids on the rate of decline in FEV1 in (A) all trials and (B) trials in which a high dose of inhaled steroids was given. Figure 2 Summary effect of inhaled corticosteroids on the rate of decline in post-bronchodilator FEV1 in patients with COPD. The centre of the diamond indicates the summary effect and its width the 95% confidence interval. Figure 3 Summary effect of high dose inhaled corticosteroids on the rate of decline in post-bronchodilator FEV1 in patients with COPD. The centre of the diamond indicates the summary effect and its width the 95% confidence interval.