CLS1113IntroductiontoClinicalLaboratoryPractices.pptVIP

CLS 1113Introduction to Clinical Laboratory Practices Unit 5 Labeled Immunoassays Chapter 10 Labeled Immunoassays Designed for Ags and Abs that DO NOT react in precipitation or agglutination tests due to their small size or low concentrations. Indirect method of detection: Competitive vs. Non-Competitive Test Antigen or Antibody competes for binding sites Elements of Labeled Immunoassays Ligands Antibodies Standards or Calibrators Separation Methods Detection of Label Radioimmunoassay (RIA) Competitive binding assay Uses a radioactive substance as a label 3H - Tritiated hydrogen 125I