[NCBI如何查找序列.docxVIP

请练习在GenBank获得一个GenBank序列文件，解释以一下各个部分代表的含义。开Genbank主页（/Web/Genbank/），②database选择“Nucleotide”，输入“Monkey（猴子）“，点击“Search”单击“search”后得到以下页面：然后选择第一个序列文献打开；找到如下图的位置，选择“file“，保存到电脑。序列内容如下：序列内容各部分意思如下：Go to:CommentFeaturesSequenceLOCUS AGMSV40RP 436 bpDNA linear PRI （名称）（碱基数）（分子类型）（分子类型）（灵长类）27-APR-1993（修正日期）DEFINITION African green monkey SV40 homologue replication origin.（定义行，精要描述序列特征）ACCESSION K01786（检索号）VERSION K01786.1 GI:176550（版本信息）KEYWORDS origin of replication.（关键词）SOURCE Chlorocebusaethiops (Cercopithecusaethiops)（物种来源及常用名）ORGANISM Chlorocebus aethiops（科学命名及该物种的分类地位）Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;Catarrhini; Cercopithecidae; Cercopithecinae; Chlorocebus.REFERENCE 1 (bases 1 to 436)（参考文献）AUTHORS Queen,C., Lord,S.T., McCutchan,T.F. and Singer,M.F.（作者）TITLE Three segments from the monkey genome that hybridize to simian（题目） virus 40 have common structural elementsJOURNAL Mol. Cell. Biol. 1 (12), 1061-1068 (1981)（期刊来源）PUBMED 6287216（数据库编号）REFERENCE 2 (sites)（参考文献）AUTHORS Dynan,W.S., Saffer,J.D., Lee,W.S. and Tjian,R.（作者）TITLE（题目） Transcription factor Sp1 recognizes promoter sequences from themonkey genome that are simian virus 40 promoterJOURNAL Proc. Natl. Acad. Sci. U.S.A. 82 (15), 4915-4919 (1985)（期刊来源）PUBMED 2991898（数据库编号）COMMENT（注释）Original source text: African green monkey liver DNA, clonepCaOri7.01 [1]. [2] sites; Sp1 binding sites. Draft entry and clean copy sequences for [2] kindly provided byW.Dynan, 31-OCT-1985. Two segments of monkey DNA were hybridized and compared to SV40DNA. Each contained multiple copies of the sequence gggcggrrwhich also appears six times near the origin of SV40 [1]. Bothcontained a long internal degenerate repeat [1]. The SV40 origin ofreplication contains several long repeats [1]. The SV40 hybridizingsegments are members of a larger family