2神经节苷脂M1介导神经生长因子..doc

神经节苷脂M1介导神经生长因子对运动 神经元再生的影响 张引成1 王贵和2 张政华1 1.西安交通大学口腔医学院口腔颌面外科, 2.安徽省铜陵市人民医院 摘　要:目的　研究神经生长因子(N GF) 、神经节苷脂M1 ( GM1) 及N GF 和GM1 混合液对桥接面神经的硅胶管腔中面神经管状化移植再生的影响。　选择成年大白兔40 只,4 只作正常对照,其余36 只随机分为A、B、C 三组,将其双侧面神经下颊支与腮腺前缘相同平面造成8 mm长缺损,用115 mm 的硅胶管进行桥接,然后作以下处理:A 组,左侧硅胶管内加入N GF ,右侧加入生理盐水;B 组,左侧加入N GF + GM1 ,右侧加入N GF ;C 组,左侧加入N GF + GM1 ,右侧加入GM1。于术后20 周进行电生理及组织学观察。　A 组左右侧神经传导速度差异无显著性;B、C 两组左右侧间差异有显著性( P 0105) 。且两组左侧再生神经有髓纤维数目、直径、髓鞘厚度、轴突直径均高于对侧,差异有显著性( P 0105) ,但A 组以上指标均无差异。光镜及电镜观察试验各组均有较明显的神经再生。　①N GF 能够促进运动神经元再生,但这种能力有限; ②GM1 能够介导N GF 促进运动神经元再生,表现出良好的生物学效应。 面神经缺损;神经再生;神经生长因子;神经节苷脂The effect of motor nerve regeneration under GM1 giving guidance to NGF ∥Zhang Yi ncheng , Wang Guihe , Zhang Zhenghua ∥Department of Oral Maxillofacial Surgery ,School of Stomatology ,Xi’an Jiaotong University ,Xi’an 710061 ,China ABSTRACT:Objective 　To study the effect s of motor nerve regeneration under GM1 giving guidance to N GF. Methods 　Fourty health adult rabbit s were used in this study ,four rabbit s were selected as normal cot rolls ,the other of thirty2six rabbit s were divided into three groups randomly. 8 mm defect s was created in bilateral facial nerve (inferior buccal branch) at the same level of the f ront border of as follow : Group A ,N GF in silicone tube of left side ,N. S in right as cont rols. Group B ,N GF + GM1 in left ,N GF in right . Group C ,N GF + GM1 in left . Regeneration of the facial nerve for all animals were examined elect rophysiologically and histologically 20 weeks after surgery. Results 　In group B and C ,the statistical difference of nerve conduction velocity (NCV) in the two sides was significant ( P 0. 05) . In group A ,NCV in N GF side was faster than that in N. S side , but no significant difference between the two sides was found. In group B and C , the number ,diameter ,myline sheath thicken and axonal diameter of the regenerating myelinated nerve in the left was greater than those in the right ,significant difference was found ( P 0. 05) . For