全自动化学发光测定仪 化学发光测定与ELISA测定AFP.doc

全自动化学发光测定仪 化学发光测定与ELISA测定AFP JournalofPharmaceuticalAnalysis2012;2(2):130–135 ContentslistsavailableatScienceDirect JournalofPharmaceuticalAnalysis /locate/ ORIGINALARTICLE Comparisonofchemiluminescenceenzymeimmunoassaybasedonmagneticmicroparticleswithtraditional colorimetricELISAforthedetectionofseruma-fetoprotein Qian-YunZhanga,b,HuiChena,ZhenLina,Jin-MingLina,n a BeijingKeyLaboratoryofMicroanalyticalMethodsandInstrumentation,DepartmentofChemistry,TsinghuaUniversity,Beijing100029,Chinab InstituteofBiophysics,ChineseAcademyofSciences,Beijing100101,ChinaReceived18August2011;accepted28October2011Availableonline27January2012 KEYWORDSa-Fetoprotein;Hepatocellularcarcinoma; Chemiluminescenceenzymeimmunoassay;Magnetic microparticles;Colorimetricenzyme-linked immunosorbentassay AbstractAchemiluminescenceenzymeimmunoassaybasedonmagneticmicroparticles(MmPs-CLEIA)wasdevelopedtoevaluateseruma-fetoprotein(AFP)inparallelwithtraditionalcolorimetricenzyme-linkedimmunosorbentassay(ELISA).AsystematiccomparisonbetweentheMmPs-CLEIAandcolorimetricELISAconcludedthattheMPs-CLEIAexhibitedfewerdosagesofimmunoreagents,lesstotalassaytime,andbetterlinearity,recovery,precision,sensitivityandvalidity.AFPwasdetectedinfortyhumanserumsamplesbytheproposedMPs-CLEIAandELISA,andtheresultswerecomparedwithcommercialelectrochemiluminescenceimmunoassay(ECLIA)kit.Thecorrelationcoef?cientbetweenMPs-CLEIAandELISAwasobtainedwithR2?0.6703;however,thecorrelationbetweenMPs-CLEIAandECLIA(R2?0.9582)wasobviouslybetterthanthatbetweencolorimetricELISAandECLIA(R2?.6866). 2011Xi抋nJiaotongUniversity.ProductionandhostingbyElsevierB.V.Allrightsreserved. 1. n Introduction Correspondingauthor.Tel./fax:?61062792343. E-mailaddress:jmlin@(J.-M.Lin). 2095i抋nJiaotongUniversity.ProductionandhostingbyElsevierB.V.Allrightsreserved. PeerreviewunderresponsibilityofXi抋nJiaotongUniversity. doi:10.1016/j.jpha.2011.10.001 Enzymeimmunoassay(EIA)isakindofimmunoassaythatusesenzymelabeledantibody/antigentodetectantige