水稻植株及其內生微生物磷脂脂肪酸生物标记异质性.docVIP

水稻植株及其内生微生物磷脂脂肪酸生物标记异质性 (福建省农业科学院，福州市，350003) 【研究目的】水稻植株和水稻内生微生物体内都含有大量的磷脂脂肪酸，利用磷脂脂肪酸分析水稻内生微生物时，内生微生物磷脂脂肪酸样本提取是连同植物体一起研磨，磷脂脂肪酸含量包含着来源于植物和微生物磷脂脂肪酸，本研究主要目的就是通过水稻组培苗磷脂脂肪酸的分析，排除植株体磷脂脂肪酸对其内生微生物的磷脂脂肪酸的干扰。【方法】试验以兴优制5、兴优HK02、准优HK05、准优航653和水稻组培苗为材料，采用改良磷脂脂肪酸法分析水稻植物自身磷脂脂肪酸和其内生微生物磷脂脂肪酸生物标记异质性。【结果】试验结果表明，水稻植株和其内生微生物共有磷脂脂肪酸有直链磷脂脂肪酸20:0、12:0、16:0、18:0和支链磷脂脂肪酸如18:3ω6с(6,9,12)、19:0 CYCLOω8с、15:1ω6с等，且共有磷脂脂肪酸在水稻中含量均低于30mg/g，共有磷脂脂肪酸在内生微生物中的含量高于30mg/g。水稻内生微生物独有磷脂脂肪酸主要为支链磷脂脂肪酸，如甲基磷脂脂肪酸，羟基磷脂脂肪酸等，共有22种如i20:0、20:1 ω9с、12:0 3OH等，含量差异很大，为0.91mg/g-11481.25mg/g。对水稻内生微生物独有磷脂脂肪酸进行对应分析CA得到，遗传距离近的水稻品种其对应距离也近，CA载荷图显示内生微生物磷脂脂肪酸对应距离小于的为多元分布，即该磷脂脂肪酸分布在多个水稻品种中；大于的为单一分布，即仅在特定的水稻品种中分布。【结论】水稻植株及其内生微生物磷脂脂肪酸异质性水稻植株和其内生微生物共有磷脂脂肪酸在水稻中含量均低于30mg/g，在内生微生物中的含量高于30mg/g。内生微生物独有磷脂脂肪酸主要为支链磷脂脂肪酸，有单一和多元分布两种，并与水稻品种有关。 Heterogeneity of fatty acids contained in the plants from rice and their endophytes HU Gui-ping, LIU Bo *, ZHENG Xue-fang, ZHANG Jian-fu, XIE Hua-an * （Fujian Academy of Agricultural Sciences, Fuzhou350003, P. R. China） Abstract: 【OBJECTIVE】The fatty acids were existed both in the rice plant and the rice endophytes. The research aim was to distinguish the fatty acids in the rice endophtes from that in the rice plant.【METHOD】The distribution characteristics of fatty acids contained in the plants from rice and their endophytes were analysised by the modified detecting method with four hybrid rices named in xingyouzhi5、xingyouHK02、zhunyouHK05、zhunyouhang653 and a rice tissue culture plantlet as a control.【RESULTS】The results revealed that fatty acids of straight chain fatty acid such as 20:0、12:0、16:0、18:0 and unsaturated fatty acids such as 18:3ω6с(6,9,12)、19:0 CYCLOω8с、a17:0、i16:1 H、16:0 N ALCOHOL、15:1ω6с、i15:0 3OH were found both in the rice plants and the rice endophytes, however, the amounts of the fatty acid in the rice plant were all less than 30mg/g compared to that of more than 30mg/g in the rice endophytes. The other 22 fatty acids including iso-, anteiso-, methyl- branched and hydroxy fa