熒光光度法测定水中硫化1.docVIP

Study on spectrofluorimetric determination of sulphides in water Zhao Baowei, Jiang Bing, Dong Wenjuan, Zhu Kun (School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China) Abstract A novel spectrofluorimetric determination method of trace amounts of sulphides was described on the basis of Hg (II)-2-(2-hydroxylphenyl) benzoimidazole (HPBI) fluorescence quenching system. The fluorescence intensity was linear with the concentration of sulphide in the range of 1.0×10-8 - 9.5×10-6 mol/L. The detection limit was down to 9.02×10-9 mol/L. Interferences was avoided by using standard distillation procedure. The main advantages, apart from the extremely high sensitivity of the method, were the high stability of the reacted sulphide system. The method was also applied to measure the trace amounts of sulphides in water sample with satisfactory results. Keywords 2-(2 -hydroxylphenyl)benzoimidazole, mercury (II), spectrofluorimetry, sulphide 1. INTRODUCTIONThe extreme toxicity of hydrogen sulphide is produced through the great ability of sulphide ion reacting with many metals in the human metabolism. Sulphides in water are oxygen demand substance, which consume the dissolved oxygen and can restrain the activity of aquatic creature [1]. The water containing sulphides causes the roots of plant to decay by irrigation. The increase in the concentration of this species in water is mainly attributed to the indiscriminate discharge of inadequate treated effluents of organic matter from industrial wastes as well as the bacterial reduction of sulphate results in the release of sulphide into wastewater. The toxic nature of H2S has claimed several lives, especially of those working in the sewerage systems[2,3]. Therefore, the facts mentioned above lead to a requirement for sensitive analytical methods for the determination of sulphides. The quantitative determination of sulphide in different types of samples has been reported by employing a rang