分子生物学全剖析.ppt

Killing Messengers Double stranded RNA mediates the silencing of the translation of its corresponding mRNA This structure is characteristic of an RNase III like cleavage pattern, which led to the identification of the highly conserved Dicer family of RNase III enzymes as the mediators of dsRNA cleavage. Are there endogenous siRNAs? Interestingly, endogenously expressed siRNAs have not been found in mammals. However, the related micro(mi)RNAs have been cloned from various organisms and cell types. miRNAs are short RNA species, about 23nt in longth, which are produced by Dicer cleavage of l