人骨髓间充质干细胞体外支持脐血cd34+细胞增殖的研究.docVIP

人骨髓间充质干细胞体外支持脐血CD34+细胞增殖的研究 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 作者：马俐君，高磊，周虹，邱慧颖，胡晓霞，解琳娜，王健民 【摘要】nbsp; 为了研究人骨髓间充质干细胞（MSC）作为滋养层细胞体外支持脐血CD34+细胞扩增的作用，将MSC和胎儿骨髓来源的成纤维细胞样骨髓基质细胞系（HFCL）经60Co γ线照射后分别制备细胞滋养层，比较不同滋养层细胞和添加或不加细胞因子对脐血CD34+细胞增殖数及LTC- IC数的影响。结果表明， 无论有无添加细胞因子(rhFL、rhSCF、rhTPO)， HFCL滋养层组培养12天扩增细胞数明显高于MSC滋养层组，以第0天细胞数为100%（下同），有细胞因子组为(9797±361）% vs (7061±418）%，无细胞因子组为(5305±354)% vs (1992±247)%，均Plt;0.01。 MSC滋养层组CD34+细胞扩增数与HFCL滋养层组相比无显著差异［（825±305）% vs (820±191)%，Pgt;0.05］，但在细胞因子存在时低于HFCL滋养层组［（939±212）% vs (1617±222)%，Plt;0.01］。MSC滋养层组维持脐血中LTC- IC的能力明显优于HFCL滋养层组［第5周CFU- GM数（129.95±8.73）个/105接种细胞数 vs （89.81±10.29）个/105接种细胞数，Plt;0.05］；细胞因子存在时，其作用更为明显［第5周CFU- GM数（192.93±4.95）个/105接种细胞数 vs （90.47±14.28）个/105接种细胞数，Plt;0.01］。MSC与HFCL按一定比例混合，可提高扩增效率。当MSC与HFCL之比为4∶1时，集落形成数量最高，达（186.89±11.11）个/105接种细胞数，明显高于比例为3∶2（131.45±13.02）个/105接种细胞数和二者单用组［前者（138.92±14.84）个/105接种细胞数，后者（64.63±6.11）个/105接种细胞数；均Plt;0.01］。结论: MSC维持脐血中LTC- IC集落形成的能力优于基质细胞系HFCL，HFCL支持脐血CD34+细胞的增殖能力优于MSC，MSC加上适量HFCL可显著提高CD34+细胞扩增效率。 【关键词】nbsp; 间充质干细胞；脐血CD34＋细胞；CD34+细胞扩增 　　Effects of Human Mesenchymal Stem Cells and Fibroblastoid Cell Line as Feeder Layers on Expansion of Umbilical Cord Blood CD34+ Cells In Vitro 　　AbstractTo investigatenbsp; the effects of human mesenchymal stem cells (MSC) and human fibroblastoid cell line (HFCL) as feeder layer on expansion of umbilical cord blood CD34+ cells in vitro,nbsp; 60Conbsp; γ-raynbsp; irradiated MSC and HFCL were used as feeder layer to expand cord blood CD34+ cells in culture. The efficiencies of MSC and HFCL on expansion of CD34+ cells in culture with or without cytokinesnbsp; were compared. The results showed that no matter whether cytokines （rhFL,rhSCF,rhTPO) were added, the proliferation of nucleated cells after expansion for 12 days in HFCL group was statistically higher than that in MSC group, i.e.nbsp; with cytokines (9797±361)% vs (7061±418)%; without cytokines (5305±354)% vs (1992±247)%,nbsp; when the