内皮细胞损伤、血小板活化及功能状态与2型糖尿病的相关性.docVIP

内皮细胞损伤、血小板活化及功能状态与2型糖尿病的相关性 【关键词】nbsp; 内皮损伤 　　Correlation between vascular endothelial cell injury, platelet activation and functional status in type 2 diabetes 　　【Abstract】 AIM: To investigate the vascular endothelial cell injury and the platelet activation and functional status changenbsp; in type 2 diabetes patients. METHODS: The plasma levels of von willebrand factor∶antigen (vWF∶Ag) and granule membrane protein140（GMP140）were measured by double sandwich ELISA. The adhesiveness of platelet was determined by Salzmans method. RESULTS: The plasma levels of vWF∶Ag（145.6±25.6）％，GMP140（16.8±3.5）μg/L and PAdT （80.3±5.3）％ of type 2 diabetes were significantly higher than those of normal controls（Plt;0.05）; The plasma levels of these markers in the group of type 2 diabetes with vascular complication were higher than those in the one without vascular complication（Plt;0.05）; plasma levels of GMP140 and PAdT were positively correlated with those of vWF∶Ag［（160.8±35.6）％，（18.9±5.5）μg/L，（89.5±7.8）％, r=0.446, 0.413, Plt;0.05］. CONCLUSION: Type 2 diabetes can cause vascular endothelial cell injury, platelet activation and functional status change. The plasma levels of vWF∶Ag, GMP140 and PAdT may reflect vascular pathological changes in type 2 diabetes patients to a certain extent. 　　【Keywords】 diabetes； endothelial cell injury；platelet activation；angiopathies 　　【摘要】 目的： 观察2型糖尿病患者内皮细胞损伤、血小板活化及功能状态的变化及临床意义. 方法： 利用双抗体夹心 ELISA法测定血浆血管性血友病因子抗原vWF∶Ag，血小板膜表面α颗粒膜蛋白GMP140水平；采用玻珠柱法测定血小板黏附率PAdT. 结果： 糖尿病组血浆vWF∶Ag（145.6±25.6）％，GMP140（16.8±3.5）μg/L，PAdT（80.3±5.3）％水平显著高于对照组（Plt;0.05）；有血管病变的患者血浆vWF∶Ag（160.8±35.6）％，GMP140（18.9±5.5）μg/L，PAdT（89.5±7.8）％水平显著高于无血管病变组（Plt;0.05）；相关分析显示，GMP140，PAdT水平与血浆vWF∶Ag水平显著正相关（r=0.446，0.413，P均lt;0.05）. 结论： 2型糖尿病患者均存在血管内皮损伤，并由此引起血小板活化及血小板功能状态的改变；血浆vWF：Ag，GMP140及PAdT水平在一定程度上可反映2型糖尿病患者血管病变的情况. 　　【关键词】 糖尿病；内皮损伤；血小板活化；血管病变 　　0引言 　　血管病变是糖尿病患者死亡和致残的主要原因，对糖尿病血管病变的发病机制做深入探讨，并据此采取适当的防治方法是十分必要的. 我们对糖尿病患者血浆vWF∶Ag, GMP140含量以及PAdT进行了检测，探讨糖尿