准分子激光80μm深度切削制备感光细胞片层.docVIP

准分子激光80μm深度切削制备感光细胞片层 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 作者：李根林 王 钢 王津津 陆文秀 王景昭 【摘要】nbsp; 目的nbsp; 探讨PTK切削方式下，准分子激光切削视网膜制备理想感光细胞片层所需的最佳切削深度。方法nbsp; 以新生小牛视网膜为研究对象，白明胶作载体，液态视网膜铺片法铺片，准分子激光PTK切削方式，共切削3种深度，制备48个感光细胞片层，行光镜和电镜观察。结果nbsp; 用准分子激光PTK切削方式，80μm切削深度下可以获得较好的视网膜感光细胞片层，光镜和扫描电镜均显示完整的感光细胞节段、外颗粒层、外网状层以及部分残存的内颗粒层细胞，而50μm和100μm深度则分别较浅和较深。结论nbsp; 80μm是准分子激光PTK方式切削视网膜制备感光细胞片层的适宜切削深度。 nbsp;nbsp;nbsp; nbsp;nbsp;nbsp; 【关键词】nbsp; 视网膜nbsp; 准分子激光nbsp; PTK方式nbsp; 感光细胞片层nbsp; 80μm切削深度 nbsp;nbsp;nbsp;nbsp;. nbsp;nbsp;nbsp;nbsp; nbsp;nbsp;nbsp; 【Abstract】nbsp; Objectivenbsp; To study the ideal ablation depth for harvesting sheets of photoreceptor cells from retina by using the excimer laser with PTK (phototherapeutic keratectomy，PTK) mode.Methodsnbsp; Retinas from neonatal calf，and gelatin as carrier were used.The retinas were located firstly and then laid on the surface of gelatin carrier using the devised liquid-phase method.The PTK mode of excimer laser was used to ablate retinal sheet.Total 48 photoreceptor sheets were ablated with three depths and were studied by light and scanning electron microscope.Resultsnbsp; With 80 micron depth of ablation，photoreceptor cells were harvested as intact sheets from the retinas using the excimer laser.Light and scanning electron microscope demonstrated the sheets of photoreceptor cells with intact outer and inner segments，outer nuclear layer，outer plexform layer and some remained inner nuclear layers.However，50μm and 100μm were found to be either rather superficial or deeper than that of 80μm.Conclusionnbsp; For harvesting ideal intact photoreceptor cell sheets，80 micron would be the most suitable depth of ablation by using the PTK mode of excimer laser. nbsp;nbsp;nbsp; nbsp;nbsp;nbsp; 【Key words】nbsp; retinanbsp; excimer lasernbsp; PTK modenbsp; photoreceptor sheetsnbsp; 80 micron ablation nbsp;nbsp;nbsp; nbsp;nbsp;nbsp; 制备理想的感光细胞片层是获得视网膜移植良好手术效果的前提之一。目前制备感光细胞片层的方法有多种，包括准分子激光切削法［1，2］、振荡切削机切片法［3］等。其中准分子