雞冠花黄酮化合物对DM小鼠脾脏及巨噬细胞吞噬功能的影.docVIP

编号：04435 鸡冠花黄酮化合物对DM小鼠脾脏及巨噬细胞吞噬功能的影响* 郭晓玲1 李万里2 尉辉杰1 张霞1 李喜龙 1 1新乡医学院基础医学院2002级临床医学专业（新乡医学院，453003） 2新乡医学院微生物与免疫教研室（新乡，453003） 摘要 目的 探讨鸡冠花黄酮类化合物对糖尿病动物模型(DM)小鼠脾脏及巨噬细胞吞噬功能的影响. 方法 选取小鼠27只，随机分为3组：(1) 正常对照（Control）组；(2)糖尿病模型(DM)组；(3)糖尿病＋鸡冠花黄酮化合物预防(CCF)组。DM组与CCF组腹腔单剂量注射链脲佐菌素（STZ）65mg/kg,制成糖尿病mg·kg -1 ·d-1）灌胃，ontrol组仅给予等量自来水灌胃 结果： CCF组与DM组相比，体重升高，而脾重指数显著降低(P0.05),单核巨噬细胞对鸡红细胞的吞噬率及吞噬指数有所降低(P0.05)，而且各项指标与Control组接近（无显著性差异）。结论：补充鸡冠花黄酮类化合物可调节糖尿病动物巨噬细胞吞噬的作用，可能有利于减少巨噬细胞激活所引起的免疫病理损伤，可用于糖尿病的预防。 关键词 鸡冠花黄酮 吞噬指数 糖尿病 *新乡医学院大学生科研重点立项课题（2004） 作者：郭晓玲（1983— ）女，河南安阳人，系新乡医学院基础医学院临床医学专业02级学生；李万里（1956— ）男，医学硕士，教授，主要从事糖尿病的发病机理与临床研究 Effects of Celosia Cristata Flavonoid on the Spleen and the Function of Macrophage Ph agocytosing in DM Mice Guo Xiaoling1 Li Wanli2 Wei Huijie1 Zhang Xia1 Li Xilong 1 1Department of 2002, the school of Basic Medical science,XinXiang Medicalcollege 2Department of Microbiology and Immuniology，XinXiang Medical College ( XinXiang ,453003) ABSTRACT Objective To study effect of the abstraction of the celosia cristata flavonoid on the spleen and the function of macrophage phagocytosing in DM mice. Methods Choose 36 mice and divide into 3 groups at random: (1)the normal group（control）; (2) the diabete model group (DM );(3) diabete + the celosia cristata flavonid group（CCF).Inject the streptozotocin(STZ) 65mg/kg with metred dose in celiac cavity in DM group and only the same quantity of caitrate buffer in control group. The CCF group are given celosia cristata flavonoid of 100mg·kg -1 ·d-1 ,the DM froup and the Control group are only given the same quantity of water.Three groups are feeded with normal food and water. After 10 weeks , measure the spleen weight and the index of the spleen weight。 The experiment of macrophage phogocytosing measures the rate and the index. Results Compared with the DM group, the weigh