流式细胞术应用概述__培训课件.pptVIP

3、流式细胞术CFSE染色分析细胞分裂增殖 Divisions: 3 2 1 0 CFSE FL-1 (Log) Divisions: 3 2 1 0 实验原理 Calculating precursor frequency 0 1590 /1 1590 1 1140 /2 570 2 1388 /4 347 3 687 /8 86 4 41 /16 2.6 Total; 2595 Cohort Total; 1005.6 % original cells dividing is 1005.6divided by (2595 + 1005.6) = 38.7% Lyons (2000) J. Imm. Meth. 243, 147 Parent: 1.22 % at 200.00 Generation 2: 2.83 % at 180.81 Generation 3: 5.67 % at 161.62 Generation 4: 8.48 % at 142.43 Generation 5: 7.31 % at 123.24 Generation 6: 18.99 % at 104.05 Generation 7: 35.96 % at 84.86 Generation 8: 19.43 % at 65.