毕业论文—人工蛹虫草子实体中核苷类成分的超声提取工艺研究.doc

摘 要 蛹虫草属于真菌类物质人工蛹虫草子实体具有很高的药用价值和营养价值，尤其是人工蛹虫草子实体中含有的核苷类物质它的应用十分广泛并有极高的应用价值和开发潜力。 本实验以人工蛹虫草子实体为原料，以核苷类物质的提取率为指标，比较了超声提取法、热回流提取法和微波提取法核苷类物质的提取效果，并提取溶剂实验结果表明超声提取法效果最佳。超声提取条件：液料比为40:1、提取温度80℃、、提取时间40min提取次数3次。HPLC法测定人工蛹虫草子实体腺苷和虫草素的含量关键词蛹虫草子实体；腺苷；虫草素；； Abstract As the fungus substance，the cultured mycelia of Cordyceps Militaris has a high medical and nutritional value, especially its nucleoside has been widely used. It has a high application value and development potential. The experiment regards the cultured mycelia of Cordyceps Militaris as the research material，the extraction rate of nucleoside as indicators . To investigate the method applied in the extraction of nucleoside in Cordyceps militaris mycelia．Water-extraction method，ultrasonic wave extraction and microwave extraction method were applied in the extraction of nucleoside．And selected the best extraction solvent.The experiment shows that the method of microwave extraction is the best,and the best extraction solvent is water.The experiment use the method of HPLC to determination of cordyceps militaris artificially fruit body and the content of Chinese caterpillar fungus adenosine meal.Orthogonal design was be used to assess the process conditions which influencing the extraction and separation ,and final optimized conditions for extracting nucleoside was established,then get the best condition is:Liquid material than is 40:1、the temperature is 80℃、the times is 3、the time is 40min. Keywords：Cordyceps；Adenosine；Cordycepin；HPLC；Ultrasonic extraction；目 录 摘要 1 Abstract 2 第一章 前言 5 第二章 文献综述 6 2.1 蛹虫草子实体 6 2.1.1 蛹虫草子实体的营养成分分析 6 2.1.2 蛹虫草子实体的功能作用 6 2.1.3 蛹虫草子实体的应用 7 2.1.4 蛹虫草子实体的国内外研究现状 10 2.2 蛹虫草子实体中的活性成分——核苷类物质 10 2.2.1 核苷类物质的结构与理化性质 10 2.2.2 核苷类物质的功能 11 2.2.3 核苷类物质的应用 11 2.3 核苷类物质的方法概述 12 2.3.1 超声波提取法 12 2.3.2 微波提取法 12 2.3.3 热回流提取法 12 2.3.4 溶剂萃取法 13 2.3.5 超临界CO2萃取法 13 2.3.6 膜分离法 13 2.3.7 柱层析分离法 14 2.3.8 无机盐复合沉淀法 14 2.4 核苷类物质的方法概述 14 2.4.1 高效液相色谱分离鉴定技术 14 2.4.2 薄层色谱分析技术 15 2.4.3 紫外分光光度法 15