不同工艺^加工的三叶青抗炎作用比较研究(中医药科技).docVIP

不同工艺加工的三叶青抗炎作用比较研究* 严余明 王忆黎1△ 童晔玲1 戴关海1 杨锋1 （ 浙江中医药大学·杭州 310053 1 浙江省中医药研究院·杭州 310007） 摘 要 目的：观察不同工艺加工的三叶青抗炎作用比较。方法：三叶青采用传统的水煎、饮片打粉与冻干粉三种不同工艺加工制成供试品，分别进行对二甲苯致小鼠耳廓肿胀抑制作用、对小鼠毛细血管通透性影响、对角叉菜胶致大鼠足跖肿胀抑制作用的抗炎实验。结果：三叶青冻干粉中、高剂量及饮片粉高剂量对二甲苯所致小鼠耳廓肿胀有明显的抑制作用，与水对照组比较差异显著（P0.05）。三种工艺三叶青对醋酸所致小鼠腹腔毛细血管通透性均有一定的抑制作用（抑制率为7.2%～33.3%），其中高剂量三叶青冻干粉抑制作用最为明显，与水对照组比较差异显著（P0.05）。致炎后1h三叶青冻干粉中、高剂量对角叉菜胶致大鼠足肿胀均有明显的抑制作用，与水对照组比较差异显著（P0.05）；致炎后4h三叶青冻干粉中、高剂量和三叶青水煎剂高剂量、三叶青饮片粉高剂量均有明显的抑制作用，与水对照组比较差异显著（P0.05）；致炎后6h，三叶青冻干粉中、高剂量均有明显的抑制作用，与水对照组比较差异显著（P0.05）。结论：三叶青冻干粉的抗炎作用明显优于水煎剂与饮片粉。 主题词 三叶青 冻干粉 水煎剂 饮片粉 抗炎作用 比较研究 Comparative Study on the Anti-inflammatory Effects of Different Processed Tetrastigma Hemsleyanum Diels et Gilg Yan Yuming , Wang Yili1△, Tong Yeling1 ,Dai Guanhai1, Yang Feng1 ( Zhejiang Chinese Medical University; 1Zhejiang Institute of Traditional Chinese Medicine) Abstract Objective To observe the anti-inflammatory effects of different processed Tetrastigma hemsleyanum Diels et Gilg. Methods Tetrastigma hemsleyanum Diels et Gilg were processed into lyophilized powder、water decoction and pieces powder. The anti-inflammatory action was assessed by several tests including xylene-induced ear edema in mice, HAc-induced peritoneal capillary permeability in mice and carrageenin-induced paw edema in rats. Results Compared to control group, high and ———————————————— * 浙江省科技计划项目No.2011C23038 △ 通讯作者 middle dose groups of lyophilized powder and high dose group of pieces powder could evidently inhibit xylene-induced ear edema in mice（P0.05）Tetrastigma hemsleyanum Diels et Gilg had some inhibitory effect on HAc-induced peritoneal capillary permeability in mice, the inhibition rate were between 7.2%-33.3%. High dose groups of lyophilized powder had the best effect , compared with control group（P0.05） hour after inflammation, high and middle dose groups of lyophilized powder could significantly inhibit carrageenin-induced paw edema in rats（P0.05） hour after inflammation, high and middle dose