mPEGPLANGF缓释微球的制备及体外释放实验研究.docVIP

mPEG-PLA-NGF缓释微球的制备及体外释放实验研究 刘 民1,2，张 纲1，谭颖徽1 （400037 重庆，第三军医大学新桥医院口腔科1；266071 山东 青岛，解放军401医院口腔科2） [基金项目] 国家自81271098）；重庆市自然基金CSTC2009BB5334） [通信作者] 谭颖徽，E-mail：tanyh1962@yahoo. com. cn [摘要] 目的 自行设计合成一种以骨缺损修复为目的的神经生长因子缓释微球，并进行理化性能和体外释放实验。 方法 复乳法制备mPEG-PLA-NGF缓释微球，并观察其大小形态，用ELISA法测定微球包封率及载药量，用动态透析释药法测定体外释放率。 结果 微球表面光滑圆整，球体大小较均匀。微球粒径为（74.2±21.3）μm，粒径分布范围较窄。包封率和载药量分别为（77.3±1.81）％和[(2.13±0.24)×10-5]%，体外释放实验中，没有发现突释现象，24h释放率为27.36％，微球在21d后释放度达72.34％。结论 mPEG-PLA-NGF缓释微球具有良好的物理特性和很好的缓释效果。 [关键词] 微球；缓释；神经生长因子；单甲氧基聚乙二醇聚乳酸；体外释放 [中图法分类号] [文献标志码] A Study on preparation of nerve growth factor mPEG-PLA sustained release microspheres and sustained release characteristics in vitro Liu Min1,2, Zhang Gang1, Tan Yinghui1 (Department of Oral and Maxillofacial Surgery,Xinqiao Hospital,Third Military Medical University.Chongqing, 400037,China) [Abstract] Objective To study the preparation and characteristic of biodegradable mPEG-PLA microspheres as carriers for controlled NGF delivery, and the sustained release rate in vitro. Methods MPEG-PLA was utilized as delivery material to prepare the NGF microspheres by multiple emulsion method. The physical surface of microspheres were detected, and the content of NGF in microspheres was evaluated by ELISA. Dialysis method was used to examine the in vitro drug release. Results The microspheres were spherical in shape, and had a smooth surface. The particle size was well-distributed with particle size of (74.2±21.3)μm，Drug-loading amount was[(2.13±0.24)×10-5]% and the entrapment rate was（77.3±1.81）%. There was no burst release in vitro.The release rate was about 27.36% of total amount in 24 hours. The accumulative sustained release rate was 72.34% in three weeks. Conclusion The mPEG-PLA-NGF microspheres had a good physical performance and sustained release character in vitro. [Key words] microspheres; controlled release; nerve growth factor; mPEG-PLA; release in vitro Supported by the General Program of Nati