planttissuecultureandmicropropagation（11页）.docVIP

STRUCTURE OF THE COURSE I TERM IN ACADEMIC INSTITUTE ( 6 MONTHS) NO. NAME OF THE PAPER HOURS/WEEK CREDITS PTC 1: PLANT TISSUE CULTURE AND MICROPROPAGATION 4 4 PTC 2: ADVANCED TECHNIQUES IN PLANT TISSUE CULTURE 4 4 PTC 3: PLANT DEVELOPMENT, BREEDING AND TRENDS IN BIOTECHNOLOGY 4 4 PTC 4: LAB TO LAND 4 4 LAB 1 PRACTICAL 1 4 hrs x 2session 4 LAB 2 PROJECT RELATED TO PRACTICAL TECHNIQUES 4 hrs x 2 session 4 24 II TERM IN THE INDUSTRY (6 MONTHS) TO BE DECIDED BY THE INDUSTRY 24 TOTAL NUMBER OF CREDITS 48 PTC 1: PLANT TISSUE CULTURE AND MICROPROPAGATION THEORY TOTAL : 40 HRS UNIT 1 INTRODUCTION TO PLANT TISSUE CULTURE Definition, brief history, principle and significance of tissue culture; Cellular totipotency – Cytodifferentation: factors affecting vascular tissue differentiation, cell cycle and TE differentiation; Organogenic Differentation: induction, factors affecting shoot bud differentiation. 4 UNIT 2 LABORATORY ORGANIZATION: Design and layout for wash area, media preparation, sterilization and storage room, transfer area for aseptic manipulations, Culture rooms, observation/data collection areas. Labwares Good laboratory practices, Good safety practices. 2 UNIT 3 INSTRUMENTATION AND STERILIZATION TECHNIQUES Working principle, maintenance and management of following instruments: Laminar air flow, autoclave, distillation unit, pH meter, orbital shaker, microscope, deep freezer, growth chamber Sterlization: Importance, Methods of sterilization of equipments, culture media and explants. 4 UNIT 4 TISSUE CULTURE MEDIA Intro