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THE LIGHT INDEPENDENT REACTION ? 2016 Paul Billiet ODWS THE LIGHT INDEPENDENT REACTION Enzyme controlled (Q10 ? 2) Located in the stroma of the chloroplast Occurs simultaneously with the light dependent reaction It can continue in the dark provided the necessary raw materials are available (CO2, NADPH + H+ and ATP). ? 2016 Paul Billiet ODWS Enzyme controlled reaction pathways A B C D E F Ea Eb Ec Ed Ee X = Precursor (raw material) added here ? 2016 Paul Billiet ODWS Enzyme controlled reaction pathways To find out the sequence of the reactions and the point at which X is added in, two