ppt课件-dnamicroarray-sunyoneonta.pptVIP

DNA Microarray: A Recombinant DNA Method Basic Steps to Microarray: Obtain cells with genes that are needed for analysis. Isolate the mRNA using extraction buffer. Remove the buffer with RNA. The mRNA will be 5’-3’ with repeating AAA..end. Convert each mRNA into colored cDNA. Targets labeled with fluorophores. Basic Steps to Microarray: With mRNA degraded reverse cDNA created. Mix colored samples of cDNA. Microarray or DNA Chip: Every spot on the chip represents a different coding sequence from different genes. Each spot on the chip is made of a DNA probe that can pair with the cDNA that was