消化科开题报告[精选].pptVIP

Tim3，即T细胞免疫球蛋白及粘附素结构域蛋白3 表达在Th1细胞表面，是其标志分子 与其配体Galectin9结合致Th1细胞减少，调节免疫反应 材料及方法 试验动物: Tim-3 transgenic mice Galectin-9 transgenic mice Tim-3?/? mice Rag1?/? C57BL/6 mice:无T和B细胞 Galectin-9 transgenic and Tim-3?/? mice EAE模型：实验性自身免疫性脑脊髓炎injected subcutaneously with 100 μg of MOG 35–55 peptide (MEVGWYRSPFSRVVHLYRNGK) emulsified in CFA (Difco) supplemented with 4 μg ml?1 Mycobacterium tuberculosis and injected twice intravenously with 100 ng of pertussis toxin (List Biological Laboratories). Notes CD44highCD62Llow：effector/memory T cell CD44lowCD62Lhigh ：naive cell CD11b+ ：monocyte/macrophage and granulocyte F4/80 : monocyte/macrophage and eosinophil Gr-1 : inflammatory monocyte and granulocyte Ly-6G and Ly-6C : CD11b+ cell TNP-OVA : 三硝基苯酚-卵清蛋白，为抗原，具有刺激免疫反应作用 Because Gr-1 antigen consists of the epitopes from both Ly-6G and Ly-6C , so they examined these two moleculars with specific antibodies and they found Tim3 Tg mice had more CD11b+ cells that were Ly-6G+ F4/80low Ly-6Clow Conclusion Tim-3/galectin-9 pathway regulates Th1 immune responses through at least two mechanisms: directly, by triggering cell death in Th1 cells indirectly, through the expansion of suppressive CD11b+ Ly-6G+cells. Galectin9转基因小鼠实验 Wild Gal9Tg 淋巴结 脾脏 CD11b+ 抗CD3 Tcell 增殖反应 分泌反应 APC B细胞 Effector/ Memory Ly-6G+ F4/80low TNP-OVA刺激 TNP-OVA刺激 How could both Tim3 and its ligand galectin9 independently result in the expansion of CD11b+Ly-6+F4/80low cells CD11b+Ly-6+ CD11b+Ly-6- Control IFN-g 检测Galectin9 Whether a direct interaction of Tim3 with galectin9 is responsible for the expansion of CD11b+Ly-6+F4/80low cells Wild 淋巴结 脾脏 Tcell 增殖反应 分泌反应 Effector/ Memory Ly-6G+ F4/80low TNP-OVA刺激 TNP-OVA刺激 Gal9Tg Gal9Tg Tim3-/- CD11b+ Valerie Dardalhon et al, J Immunol ,2010 Tim-3/galectin-9 pathway: regulation of Th1 immunity through promotion of CD11b Ly-6G myeloid cells 研究背景 分泌IL-2、IFN-γ、TNF 介导细胞免疫 Th Th1 Th2 Th17 Treg 分泌IL-4、IL-5、I