GHR在腔粘连患者子宫内膜组织中的表达及意义.docVIP

GHR在患者子宫内膜组织中的表达及意义摘 要 目的: 探讨生长激素受体（GHR)在患者子宫内膜组织中的表达及意义。方法: 通过免疫组化半定量和实时荧光定量PCR（QPCR）两种实验方法分别检测组（研究组）和非组（对照组）患者子宫内膜组织GHR蛋白和mRNA的表达情况结果免疫组化GHR蛋白：研究组1.70±0.57 对照组2.77±0.75 P =0.00；QPCR实验方法 GHRmRNA表达：研究组0.64±0.21，对照组1.00±0.33，P=0.03差异有统计学意义。GHR蛋白表达χ2=6.053，P=0.025GHR蛋白比较差异有统计学意义。结论患者子宫内膜组织中GHR的表达低于非者，表达子宫内膜腺体中较多，在子宫内膜间质中较少为IUA患者临床使用生长激素 【关键词】 生长激素受体 生长激素 Expression and significance of Growth hormone receptor in theendometrial tissue of intrauterine adhesion patients Ren Juan, Wei Bing, Song Enxue, et al (Dept of Gynecology and Obstetrics，The Second Affiliated Hospital of Anhui Medical University，Hefei 230601)Abstract Objective To investigate the growth hormone receptor (GHR) expression and significance of endometrial tissue in patients with intrauterine adhesions . Methods Semi-quantitative immunohistochemistry and real-time quantitative PCR (QPCR) were used to detect the expression of GHR protein and mRNA IUA in patients endometrial tissue of IUA group (study group) and non IUA group (control group) . Results The immunohistochemical expression of GHR experimental methods: The study group 1.70 ± 0.57, control group 2.77 ± 0.75, P = 0.00; QPCR experimental methods GHRmRNA expression: The study group 0.64 ± 0.21, control group 1.00 ± 0.33, P = 0.03, two group differences were statistically significant. Immunohistochemistry methods: endometrial glands and endometrial stromal GHR protein expression χ2 =5.883, P = 0.025, two parts of the GHR protein expression difference was statistically significant. Conclusion The expression of GHR in endometrial tissue in patients with IUA is lower than those of non-IUA, and this expression is more in endometrial glands, less in endometrial stroma ,which provide a theoretical reference for the use of hormone in IUA patients. Key words intrauterine adhesions; growth hormone; growth hormone receptor 宫腔粘连(Intrauterine adhesions，IUA) 是因为子宫内膜基底层受损导致肌层裸露,创面的炎性趋化因子和胶原蛋白渗出沉积 再加上宫腔各壁创面紧密接触,在内膜逐渐修复的过程中形成纤维粘连带，导致宫腔形态和子宫内膜的生理功能难以恢复。临床主