微藻培养过程中一氧化二氮的定量测定讲解.ppt

Quantitative Measurement of Direct Nitrous Oxide Emissions from Microalgae Cultivation Life Cycle Assessments (LCA) of microalgae-based biofuels have suggested net reductions of greenhouse gas emissions a kind of potent greenhouse gas isnt considered in LCA BACKGROUND METHODS AND METERIALS Cultivate the microalgae with light-dark cycling 2 1 3 Simulate PBR (photobioreactor) and ORP (open raceway pond) conditions Using Fourier transform infrared(FTRI) mearsure N2O quantitatively Microalgae species Nannochloropsis salina (1776) 4 DNA extraction and PCR amplification RESULTS ORP Condition The total emission mass of N2O 2.35×10-5kg ORP Condition RESULTS PBR Condition The total emission mass of N2O 4.38×10-4kg PBR Condition N2O production route？？？？？ Hypothesis： RESULTS Denitrifying Bacteria norB genes was detected in the experiment cultures encoding for nitric oxide reductase, which catalyzes the reduction of NO to N2O N2O emissions observed in these studies are a byproduct of the anoxic bacterial denitrification pathway RESULTS SUMMARY 1.Using FTIR measure the N2Oemission, get the quantitative date of N2O emmision 4.N2O emissions are a byproduct of the anoxic bacterial denitrification pathway 5.Based on the data presented in this study, the LCAfor microalgae LCA in the future must consider the potential for direct N2O emissions from the cultivation stage 2.Light periods were significantly lower than the emissions for the dark periods both in PBR and ORP condition 3.Cumulative N2O emissions in the PBR is substantially higher than the measured in the ORP under the same conditions. Thank You! QA