疟原虫镜检技术解析.pptVIP

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * 2.疟原虫的厚薄血片都必须在油镜下观察，显微镜用光要充足，包括反光镜、光圈、聚光器要达到油镜使用的最佳限度。 3.如果不出现物象或者目标不理想要重找，在加油区之外重找时应按：低倍→高倍→油镜程序。在加油区内重找应按照：低倍→油镜程序，不得经高倍镜，以免油沾污镜头。 注意保护镜头，切不可压碎标本被片，损坏镜头。 4.视野模糊不清 显微镜问题： 镜头镜油用量、沾污灰尘或含有气泡； 油镜头破损、进油或有霉斑、灰尘； 显微镜用光调整不足。 血片问题： 载玻片不洁、毛玻璃样或有霉斑； 血片染色过度或血膜过厚。 5.对熟练的镜检员一般以镜检厚血膜为主,以提高阳性检出率，薄血膜可作虫种鉴定及形态识别之用。 6. 油镜使用完毕，需用擦镜纸沾取少量二甲苯将油擦去，并将残留的二甲苯擦 7.使显微镜的各部件恢复回原位，下降镜筒，使物镜呈“八”字形置于载物台上； 8 显微镜应存放在干燥阴凉的地方，不要放在强烈的日光下暴晒，如长时间不用，则光学部分应卸下放在干燥器中，以免受潮生霉； 镜检示教 以下血片采自现症病例 This is a thin film from a 27 year old female backpacker, with a recent history of trekking through Northern Thailand and high fever. A typical Plasmodium falciparum presentation. ●Numerous fine ring forms ●Double chromatin dots ●Marginal forms ●Red cells are not enlarged A thin film from a 22 year old male holidaying in Lombok (Indonesia) one month previously. Intermittent fevers since returning. A typical Plasmodium vivax presentation ●Salient features are: ●Developing and thick (signet) ring forms ●Enlarged red cells Recent travel in Africa. Pyrexia of unknown origin. A typical Plasmodium ovale presentation Developing form of plasmodium Comet-like red cells Enlarged red cell Female patient, arrived from Brazil two weeks previously. Flu like symptoms since arrival. A typical Plasmodium malariae presentation Broad band form of plasmodium Red cells not enlarged 读片练习 2. Intermittent fever; arrived from India 2 months ago. 3. History of round the world safari including S. America, Asia and Africa. General malaise. 4. Unspecified overseas travel. Presented with temperature 38.4C 5. Male recently returned to Australia after backpacking through Asia. History of flu like symptoms. 6. New Australian previously from Malaysia. Intermittent temperatures and headaches. 7. Aches and pains, with fever. ? Glandular Fever, ?malaria. 18. Recent trip to Southern India- he