苦菜总黄酮对小鼠脑缺血缺氧保护作用的实验研究.docVIP

苦菜总黄酮对小鼠脑缺血缺氧保护作用的实验研究 【关键词】 苦菜总黄酮；,,脑缺血；,,脑缺氧；,,保护作用, 　　摘要：目的 研究苦菜总黄酮对小鼠脑缺血缺氧的保护作用。方法采用小鼠断头、结扎双侧颈总动脉等脑缺血缺氧模型，观察苦菜总黄酮对脑缺血缺氧小鼠存活时间、断头小鼠喘息时间及脑毛细血管通透的影响；TBA法、NBT法测定小鼠脑缺血缺氧后脑组织MDA含量和SOD活性。结果苦菜总黄酮能显著延长小鼠存活时间、喘息时间，能显著降低缺血缺氧小鼠脑毛细血管的通透性，能显著降低脑组织MDA含量，能显著提高脑组织SOD活性。结论 苦菜总黄酮对小鼠脑缺血缺氧有显著的保护作用，其作用机制可能与提高SOD活性，降低MDA含量有关。 　　关键词：苦菜总黄酮； 脑缺血； 脑缺氧； 保护作用 　　Study on the Protective Effect of Bitter Vegetable Flavonoids against Cerebral Ischemia and Anoxia in Mice 　　Abstract：ObjectiveTo observe the protective effect of bitter vegetable flavonoids（BVF） against cerebral ischemia and anoxia in mice.MethodsThe model of cerebral ischemia and anoxia in mice was established by decollation and ligating common bicarotid artery the effect of BVF on the survival time of the mice,and the time of catching their breath after mice decollated,and mouse brain capillary vessel permeability were observed. The content of maiondialdehyde（MDA）,activity of superoxide dismutase（SOD）in the brain tissues of cerebral ischemia and anoxia mice were measured by TBA,NBT technology respectively.ResultsBVF could remarkably lengthen the survival time of cerebral ischemia and anoxia mice, and the time of catching their breath after mice decollated,could evidently decrease mouse brain capillary vessel permeability and the content of MDA, increase activity of SOD in the brain tissues of cerebral ischemia and anoxia mice.ConclusionBVF has protective effect obviously on cerebral ischemia and anoxia in mice, its mechanism may be related to the decrease of MDA content and the increase of SOD activity content of MDA,and increase significantly activity of SOD in the mouse brain tissues of cerebral ischemia and anoxia. 　　Key words：Bitter vegetablef flavonoids； Cerebral ischemia and anoxia； Pretective effect 　　苦菜为菊科植物苦苣菜Sonchus Oleraceus L.的全草。文献［1］记载苦菜具有清热凉血、解毒消肿功能，久服可安心益气，轻身耐老。现代药理研究证实，苦菜有保肝降酶，抗心肌缺血作用［2］。本文研究旨在探讨苦菜总黄酮对小鼠脑缺血缺氧的保护作用，并探讨其药理作用机制，更好地为苦菜的临床应用提供科学理论依据。 　　1 材料 　　1.1 动物昆明种小鼠1