生长激素对梗阻性黄疸大鼠肠黏膜屏障功能损害的保护作用.docVIP

生长激素对梗阻性黄疸大鼠肠黏膜屏障功能损害的保护作用 【关键词】 重组人生长激素；胆汁淤积；黏膜屏障；免疫屏障 【Abstract】 AIM: To probe into the protective influence on the mechanical and immune barriers exerted by recombinant human growth hormone (rhGH) at ileum in rats with obstructive jaundice. METHODS: In common bile duct (CBD)ligated Wistar rats, morphological changes in ileac mucous membrane, sIgA variation in ileac juice and altered bacterial translocation were observed and compared before and after rhGH administration. RESULTS: CBDligated rats had thinner ileac mucous membrane ［(232±22) μm vs (362±43) μm］ and shorter villi ［(158±22) μm vs (254±39) μm］. Meanwhile, sIgA concentration in ileac juice dropped ［(70±14) mg/L vs (162±32) mg/L］ and bacterial translocation rate increased (3/12 vs 0/15). After using rhGH administration for 1 week, the ileac mucous membrane of CBDligated rats get thicker ［(308±34) μm vs (232±22) μm］ and the villi get longer ［(216±29) μm vs (232±22) μm］ and stronger. Microstructure of ileac membrane get comparatively normalized. sIgA level in ileac juice rose ［(114±18) mg/L vs (70±14) mg/L］. Rate of bacterial translocation decreased (2/15 vs 3/12). CONCLUSION: Rats with obstructive jaundice suffer from morphological and immune abnormalities in ileac membrane. rhGH administration can correct these abnormalities and afford a good protection on impaired mucosal and immune barriers in rats with obstructive jaundice. 【Keywords】 recombined human growth hormone; cholestasis; ileac mucosal barrier; immune barrier 【摘要】 目的：探讨重组人生长激素(rhGH)对梗阻性黄疸大鼠肠道机械屏障和免疫屏障的保护作用. 方法：结扎大白鼠胆总管并应用rhGH，观察其回肠黏膜形态学、回肠液sIgA改变及肠道细菌移位情况. 结果：梗阻性黄疸时，大鼠回肠壁呈现肌层变薄，黏膜厚度减少［(232±22) μm vs (362±43) μm］，肠绒毛变短［(158±22) μm vs (254±39) μm］,变细或绒毛融合等形态学改变；回肠液中sIgA浓度减少［(70±14) mg/L vs (162±32) mg/L］及细菌移位例数增加(3/12 vs 0/15). 应用外源性生长激素1 wk后，回肠黏膜厚度增加［(308±34) μm vs (232±22) μm］，肠绒毛增粗、增长［(216±29) μm vs (232±22) μm］，结构保持相对完整，回肠液中sIgA浓度回升［(114±18) mg/L vs (70±14) mg/L］，细菌移位减少(2/15 vs 3/12). 结论：梗阻性黄疸时，