论文-微波法芹菜中芹菜素的提取和芹菜中芹菜素的测定.docVIP

微波法芹菜中芹菜素 的提取和芹菜中芹菜素的测定 摘要：建立一种测定芹菜中芹菜素含量的紫外可见分光光度法，对芹菜的新鲜叶片、新鲜茎中芹菜素含量进行测定。采用紫外可见分光光度直接测定法，检测波长为270nm芹菜素在0.023~0.115 mg·m-1范围内线性关系良好，精密度较高，稳定性较好紫外可见分光光度法简便，快速，可用于芹菜中芹菜素含量的测定；提取时间短，提取率高，是强化固液提取过程颇具发展潜力的一项新型辅助提取技术关键词：芹菜素；紫外可见分光光度法；测定Microwave method in The celery, celery and The Extraction of Celery in The Determination of Celery Element Hu Li Jiao Liu-Yun Zhang Juan Tao qiang (Hengyang Normal College Life Science zhi dao lao shi:He Li-Fang) Abstract: Establishing a determination in the celery, content of celery ultraviolet spectrophotometry, the fresh leaf of celery, fresh meat content in the stem celery were determined. Utilizes uv-vis spectrophotometry method detected wavelength determined directly, for 270 nm, celery element in 0.023 ~ 0.115 mg · mL-1 range, high precision and good inside sex, stability is good. And the method of microwave extraction in the celery, celery, the experimental results show that: to the volume fraction of 65% ethanol concentration for material liquid extraction agents, than (g/mL) for 1:7. 5 conditions the first 2 min, the second time extracting extraction for best extraction conditions 1 min. Ultraviolet spectrophotometry is simple, fast, and can be used in the celery celery element content is determined; Microwave extraction method of short time, high yield, is to strengthen the solid and liquid extraction process has the potential for the development of a new auxiliary extraction technology. Keywords: celery; Celery element; Ultraviolet spectrophotometry; Determination; Microwave method: household microwave oven; Extraction conditions 1 前言部分 芹菜(Apium grauensL.Var.dulecdc.)为伞形科芹菜属草本植物，是我们日常生活中食用的一种蔬菜，具有“补血、祛风、健脾利湿”的作用，且能清理胃中湿浊。由于它具有一定的药用保健功能，有“药芹”之称。研究表明：芹菜中含多种化学成分，主要有芹菜素 (Apigenin)、挥发油、有机酸、胡萝卜素(Carotence)、维生素C、糖类及多种黄酮类化合物。其中具有独特的药理效应和生物学功能成分的芹菜素属于一种黄酮类化合物，分子式为C15H18O15，化学结构为4’,5,7—三羟黄酮，其4’,5,7位置的3个羟基和C2 C3双键决定了其独特的药理学效应和生物学特性。近年来大量的实验表明芹菜素具有多种药理功效：镇静、安神、保护皮质神经元、预防动脉粥样硬化、雌激素作用、抑制肿瘤血管形成、侵袭和转移、降压、抗氧化、抗菌消炎和利尿的作用。在临床上